A soybean extract fermented with koji mold and supplied from Japan as a pale yellow powder on dextrin, standardised to at least 5% 8-hydroxyisoflavones by HPLC. Fermentation turns part of the soy isoflavones into these 8-hydroxy forms, which unfermented soy isoflavones do not contain. The reference intake is 100 mg a day, and the human studies on this powder used 84 to 100 mg a day in hard capsules.
Tell us what you are developing and which documents you need. We reply from Japan within one working day with the product specification, SDS and the CoA of the current lot.
The powder starts from a soybean extract that contains isoflavones. It is fermented with koji mold: the isoflavone glycosides are split into aglycones by β-glucosidase, and the aglycones are then hydroxylated at the 8-position, giving 8-hydroxydaidzein, 8-hydroxygenistein and 8-hydroxyglycitein. The fermented extract is combined with dextrin and supplied as a pale yellow powder with at least 5% 8-hydroxyisoflavones.
Step 1 · isoflavone-containing
The starting material of the fermentation
Step 2 · koji mold
β-glucosidase splits the isoflavone glycosides into aglycones
Step 3 · hydroxylase
Daidzein, genistein and glycitein become their 8-hydroxy forms
Step 4 · dextrin
Labeling example: dextrin, fermented soybean extract
Step 5 · pale yellow powder
Characteristic odour
Step 6 · 8-hydroxyisoflavones min. 5% (HPLC)
Moisture, heavy metals, arsenic and microbiology within limits
Step 7 · 1 kg aluminium bag
Shelf life 3 years from manufacture
The grade on this page. Other grades, where available, are specified on request.
Example lot values are published in the developer's guide below. They are an example, not a specification; your lot's CoA governs.
A Japanese trading company selling directly to brand owners and contract manufacturers. No distributor layer between you and the documents.
Every shipment travels with the Certificate of Analysis of its own lot, with the measured values and the full safety panel.
A small first order lets you run a pilot batch before committing to bulk. Larger volumes are quoted by volume and delivery terms.
For registered companies developing a product. Tell us the format you plan and we will say honestly what to expect.
After payment is received. Freight quoted on request. Specification and SDS before you order, CoA with the goods.
If you are comparing ingredients for a new SKU, these questions separate one material from another quickly.
The specification fixes 8-hydroxyisoflavones at 5% or more, while total isoflavones are measured and reported for each lot. Isoflavone supplements have been assessed by food safety authorities, for example in Japan in 2006 and by EFSA in 2015 (see the guide below). Ask for the lot Certificate of Analysis and check your daily amount with your regulatory adviser.
The studies on this powder measured glycation and oxidative stress markers, skin parameters and cognitive test scores. Whether a finished product may carry any statement about them depends on the destination market. We supply study summaries; the wording is the brand owner's decision.
The powder contains soy, which is a labelled allergen in many markets. GMO status of the soybean, halal and kosher are not stated in the documents we hold. Halal documentation is often requested for supplements in the UAE; list what you need so we can check it for your order.
The EU novel food status of this fermented extract is not confirmed in the documents we hold. If you plan to sell in the EU, tell us early so the status is checked before you formulate.
The reference intake is 100 mg a day, and the human studies used hard capsules, so the powder fits one capsule or tablet next to other actives. It is a pale yellow powder with a characteristic odour; try the sample in your format before you fix the recipe.
What each component is, what the processing changes, and what published research on the raw material has examined. No health claims; references on request.
Soybeans carry isoflavones that are mostly bound to sugars. In this powder an isoflavone-containing soybean extract is fermented with koji mold, the mold behind miso and soy sauce. Fermentation frees the isoflavones from their sugars and adds a hydroxyl group at the 8-position, giving 8-hydroxyisoflavones, which unfermented soy does not contain. The powder is standardised to at least 5% of them by HPLC and carried on dextrin.
The material comes out of glycation research: the slow reaction between sugars and the body's proteins that leaves advanced glycation end products (AGEs) on long-lived proteins such as skin collagen. It has been studied in laboratory glycation assays and in human studies that measured AGE markers in blood and skin; the guide below lists what each study examined.
The reference intake is 100 mg a day, so the powder takes little room in a capsule or tablet and leaves space for other actives. That suits beauty-from-within and healthy-ageing formulas, for example next to a protein base such as Fish Collagen Tripeptide. For soy-based concepts, Fermented Soy Tempeh Powder is another fermented soybean ingredient in our range.
The minimum order is one 1 kg aluminium bag. Samples are free for registered companies developing a product. The specification, SDS and the Certificate of Analysis for your lot are confirmed with your quotation. Our own OEM line at Tomioka Plant makes finished capsules, tablets and stick packs from 5,000 units per SKU; we check the powder's fit for your format with the sample.
The notes below explain the active compounds in this powder and the research around them. They are a reference for product developers, not claims for this ingredient or for any finished product.
The main soy isoflavones are daidzein, genistein and glycitein. In soybeans they occur mostly as glycosides, bound to a sugar. When soybean material is fermented with Aspergillus oryzae, the glycosides fall and the free aglycones rise during the first 12 hours; after that, hydroxylated forms appear: 8-hydroxydaidzein, 8-hydroxygenistein and 8-hydroxyglycitein [1]. In the documents we hold for this powder the same two steps are described: β-glucosidase frees the aglycones, and a hydroxylase adds the hydroxyl group at the 8-position.
This conversion is typical of the Aspergillus molds used for fermented soy foods. When 21 fungal strains from dou-chi, miso, sake, soy sauce and sufu were screened, all Aspergillus strains (A. oryzae, A. sojae and A. tamarii) turned daidzein and genistein into 8-hydroxydaidzein and 8-hydroxygenistein, while none of the other fungi did [2]. These ortho-hydroxy isoflavones are rarely found in plants and are usually isolated from fermented soybean foods or from fermentation broths fed with soybean material [3]. 8-Hydroxydaidzein has also been produced from soybean extract with A. oryzae in a fermentor [4].
Our documents name the fermenting organism as koji mold; they do not state the species or the strain.
Glycation, or the Maillard reaction, is a non-enzymatic reaction between reducing sugars and the free amino groups of proteins and amino acids. It modifies proteins with structures such as carboxymethyllysine (CML), pentosidine and pyrraline, which are collectively called advanced glycation end products (AGEs) [5]. AGEs also form in the body during normal ageing, and long-lived proteins such as skin collagen accumulate them; their role in skin ageing is an active field of research [6]. CML and pentosidine are therefore common markers in glycation studies, including the human studies on this powder.
The technical documentation describes the following work. We list the design only; the studies are not claims for this ingredient or for a finished product.
Full descriptions, including the reported results, are available to registered companies on request.
Isoflavones in supplements are reviewed by food safety authorities. Japan's Food Safety Commission published its safety assessment of foods for specified health uses containing soy isoflavones in 2006 [7]. In 2015 EFSA assessed food supplements with isolated isoflavones for peri- and post-menopausal women; it could not derive a single health-based guidance value and noted that the doses and durations used in intervention studies could serve as guidance [8]. Total isoflavones in this powder are reported for each lot, so check your daily amount against your market's guidance. The powder contains soy. Statements about the effect of a finished product are the brand owner's responsibility under the rules of the destination market, and registration of the finished product sits with the importer or brand owner.
Sources
Start at the minimum order. Free sample. Specification, SDS and lot CoA on request.
Request a sample or quotationSoybeans carry isoflavones that are mostly bound to sugars. In this powder an isoflavone-containing soybean extract is fermented with koji mold, the mold behind miso and soy sauce. Fermentation frees the isoflavones from their sugars and adds a hydroxyl group at the 8-position, giving 8-hydroxyisoflavones, which unfermented soy does not contain. The powder is standardised to at least 5% of them by HPLC and carried on dextrin.
The material comes out of glycation research: the slow reaction between sugars and the body's proteins that leaves advanced glycation end products (AGEs) on long-lived proteins such as skin collagen. It has been studied in laboratory glycation assays and in human studies that measured AGE markers in blood and skin; the guide below lists what each study examined.
The reference intake is 100 mg a day, so the powder takes little room in a capsule or tablet and leaves space for other actives. That suits beauty-from-within and healthy-ageing formulas, for example next to a protein base such as Fish Collagen Tripeptide. For soy-based concepts, Fermented Soy Tempeh Powder is another fermented soybean ingredient in our range.
The minimum order is one 1 kg aluminium bag. Samples are free for registered companies developing a product. The specification, SDS and the Certificate of Analysis for your lot are confirmed with your quotation. Our own OEM line at Tomioka Plant makes finished capsules, tablets and stick packs from 5,000 units per SKU; we check the powder's fit for your format with the sample.
The notes below explain the active compounds in this powder and the research around them. They are a reference for product developers, not claims for this ingredient or for any finished product.
The main soy isoflavones are daidzein, genistein and glycitein. In soybeans they occur mostly as glycosides, bound to a sugar. When soybean material is fermented with Aspergillus oryzae, the glycosides fall and the free aglycones rise during the first 12 hours; after that, hydroxylated forms appear: 8-hydroxydaidzein, 8-hydroxygenistein and 8-hydroxyglycitein [1]. In the documents we hold for this powder the same two steps are described: β-glucosidase frees the aglycones, and a hydroxylase adds the hydroxyl group at the 8-position.
This conversion is typical of the Aspergillus molds used for fermented soy foods. When 21 fungal strains from dou-chi, miso, sake, soy sauce and sufu were screened, all Aspergillus strains (A. oryzae, A. sojae and A. tamarii) turned daidzein and genistein into 8-hydroxydaidzein and 8-hydroxygenistein, while none of the other fungi did [2]. These ortho-hydroxy isoflavones are rarely found in plants and are usually isolated from fermented soybean foods or from fermentation broths fed with soybean material [3]. 8-Hydroxydaidzein has also been produced from soybean extract with A. oryzae in a fermentor [4].
Our documents name the fermenting organism as koji mold; they do not state the species or the strain.
Glycation, or the Maillard reaction, is a non-enzymatic reaction between reducing sugars and the free amino groups of proteins and amino acids. It modifies proteins with structures such as carboxymethyllysine (CML), pentosidine and pyrraline, which are collectively called advanced glycation end products (AGEs) [5]. AGEs also form in the body during normal ageing, and long-lived proteins such as skin collagen accumulate them; their role in skin ageing is an active field of research [6]. CML and pentosidine are therefore common markers in glycation studies, including the human studies on this powder.
The technical documentation describes the following work. We list the design only; the studies are not claims for this ingredient or for a finished product.
Full descriptions, including the reported results, are available to registered companies on request.
Isoflavones in supplements are reviewed by food safety authorities. Japan's Food Safety Commission published its safety assessment of foods for specified health uses containing soy isoflavones in 2006 [7]. In 2015 EFSA assessed food supplements with isolated isoflavones for peri- and post-menopausal women; it could not derive a single health-based guidance value and noted that the doses and durations used in intervention studies could serve as guidance [8]. Total isoflavones in this powder are reported for each lot, so check your daily amount against your market's guidance. The powder contains soy. Statements about the effect of a finished product are the brand owner's responsibility under the rules of the destination market, and registration of the finished product sits with the importer or brand owner.
Sources
It is a soybean extract containing isoflavones, fermented with koji mold and supplied from Japan as a pale yellow powder on dextrin. Fermentation converts part of the soy isoflavones into 8-hydroxyisoflavones (8-hydroxydaidzein, 8-hydroxygenistein and 8-hydroxyglycitein), and the specification sets them at not less than 5% by HPLC. The labeling example is dextrin, fermented soybean extract, so the powder contains soy.
Laboratory work looked at the formation of AGEs such as CML and pentosidine in protein models, the cleavage of AGE-type crosslinks and radical scavenging, mostly comparing fermented with unfermented soy isoflavones. In people, a 16-week open study gave 100 mg a day to 9 healthy adults and measured plasma CML and pentosidine, skin AGE accumulation, oxidative stress markers and skin imaging; a 12-week randomised, double-blind, placebo-controlled study gave 84 mg a day to healthy adults aged 40 to 65 and used a computerised cognitive test battery. Study summaries are available to registered companies on request; they are not claims for a finished product.
Iizuka Shoukai supplies the powder with a 1 kg minimum order, packed in a 1 kg aluminium bag; the price is confirmed in your quotation. Larger orders are quoted by volume and freight is quoted on request. Goods ship from Japan 1-1.5 weeks after payment when stock is available; otherwise timing is confirmed in your quotation. Payment is 100% prepayment by SWIFT or Stripe. Iizuka Shoukai also offers OEM finished-product manufacturing in capsules, tablets and sticks at our Tomioka Plant in Gunma, from 5,000 units per SKU.
The reference intake is 100 mg a day, which gives at least 5 mg 8-hydroxyisoflavones at the 5% minimum. The human studies used 84 to 100 mg a day in hard capsules, so the powder fits one capsule or tablet next to other actives. Total isoflavones are reported for each lot; check your daily amount against your market's guidance on isoflavone intake from supplements.
