Heat-killed cells of a human-origin Enterococcus faecalis strain, dried to a light brown powder with at least 2.8 trillion cells per gram. The powder is 100% bacterial cells with no carrier, made in Japan and stored at room temperature.
Tell us what you are developing and which documents you need. We reply from Japan within one working day with the product specification, SDS and the CoA of the current lot.
The strain is grown in a starter culture and then a main culture, with pH, turbidity and a contamination test checked at every production. The cells are separated and washed by centrifugation, heat-treated, dried and sieved. The finished powder is 100% bacterial cells; the culture-medium component and processing aids are listed in the specification.
Step 1 · pH, turbidity
Contamination test at every production
Step 2 · pH, turbidity, viable count
Contamination test at every production
Step 3 · centrifugation
After a 40-mesh strainer; supernatant turbidity checked
Step 4 · centrifugation
Between 100-mesh strainers; supernatant turbidity checked
Step 5 · 100 °C, 70 min
Cells inactivated: not a live probiotic
Step 6 · inlet 200 °C, outlet 80 °C
Cells dried to powder
Step 7 · 80 mesh
Uniform powder
Step 8 · 1 kg bags
Tested against the product specification; weight and appearance checked
The grade on this page. Other grades, where available, are specified on request.
Example lot values are published in the developer's guide below. They are an example, not a specification; your lot's CoA governs.
A Japanese trading company selling directly to brand owners and contract manufacturers. No distributor layer between you and the documents.
Every shipment travels with the Certificate of Analysis of its own lot, with the measured values and the full safety panel.
A small first order lets you run a pilot batch before committing to bulk. Larger volumes are quoted by volume and delivery terms.
For registered companies developing a product. Tell us the format you plan and we will say honestly what to expect.
After payment is received. Freight quoted on request. Specification and SDS before you order, CoA with the goods.
If you are comparing ingredients for a new SKU, these questions separate one material from another quickly.
Heat-killed cells do not dissolve. In tablets, granules and capsules this does not matter; in drinks expect sediment and plan a "shake before use" instruction, as finished beauty drinks with this ingredient already do.
Color matters in white blends and clear bases. Check it with the sample in your own base.
Registration or notification of the finished product stays with the brand. Tell us the market early so we can say which documents we hold.
They are not among the documents we hold for this ingredient. Say so early if your market requires them.
The producer's documents contain no toxicology studies and set no ADI or intake limits. If your customer or regulator asks for a safety assessment, plan it before you order.
Corn is declared as non-segregated in the producer's raw material survey, so the ingredient is not offered as non-GMO.
The SDS classifies the dust as a respiratory sensitizer. Plan ventilation and respiratory protection for the people who handle the powder.
What each component is, what the processing changes, and what published research on the raw material has examined. No health claims; references on request.
Heat-Killed Enterococcus faecalis Powder is a concentrated lactic acid bacteria ingredient for brands that declare a cell count rather than a live culture. Every gram carries at least 2.8 trillion heat-killed cells and nothing else: there is no dextrin or other carrier in the powder, so the formulator decides how the cells are diluted. As a calculation, 10 mg of powder carries at least 28 billion cells; the producer sets no recommended daily intake, and the dose of a finished product is set by the brand for its market.
The producer lists tablets, granules and soft capsules for supplements, and cosmetics, where the INCI name is Enterococcus Faecalis. The powder is stored at room temperature, so it needs no cold chain. The cells do not dissolve: in liquids they settle, and beauty drinks already on the market with this ingredient carry a "shake before use" instruction on the label.
Finished products can be filled and packed at our own Tomioka Plant in Gunma. Another fermentation-based ingredient in the range is Lactic Acid Fermented Barley Extract Powder, a heat-sterilized barley extract fermented with lactic acid bacteria.
Tell us the format you plan and we will say honestly what to expect. Request the specification, a quote or a sample.
This section explains the species, the terms used for inactivated bacteria and how the cells are counted. It is a class reference: it describes the material, not the results of studies on this strain, and we make no health claims from it.
Enterococcus faecalis is a lactic acid bacterium, a round (coccus-shaped) cell that is part of the normal gut flora. Enterococci occur in high numbers in some fermented cheeses and sausages, although they are not added deliberately as starter cultures, and some Enterococcus faecalis and Enterococcus faecium strains are used as probiotics, mostly in pharmaceutical preparations (Franz et al., 2011). The ingredient on this page is a single human-origin strain of the species, supplied as heat-killed cells.
Food microbiologists assess enterococci strain by strain: some strains, mostly hospital-adapted lineages, carry antibiotic-resistance or virulence traits, and the review literature concludes that the safety of each strain used in foods has to be assured individually (Franz et al., 2011). For your dossier, ask us early for the documents you need; the specification above lists what the producer's documents contain and what they do not.
Probiotics are by definition live microorganisms. For inactivated cells two terms are in use. In 2011 Taverniti and Guglielmetti proposed "paraprobiotic" for inactivated microbial cells or cell fractions, noting that non-viable cells could remove the shelf-life problems of live cultures (Taverniti and Guglielmetti, 2011). In 2021 an expert panel of the International Scientific Association for Probiotics and Prebiotics (ISAPP) defined a postbiotic as a "preparation of inanimate microorganisms and/or their components that confers a health benefit on the host" (Salminen et al., 2021). Under that definition the word applies to a product only when a health benefit has been shown for it, so on this page we describe the ingredient simply as heat-killed lactic acid bacteria.
Live probiotics are counted in colony-forming units (CFU), which only living cells produce. Heat-killed cells cannot form colonies, so this ingredient is specified by total cell count with DAPI, a fluorescent stain that binds DNA and makes every cell visible under the microscope, intact and inactivated alike. The figure of at least 2.8 trillion cells per gram is a DAPI count. The total plate count in the specification (max. 1,000 /g) is a separate hygiene parameter for viable microorganisms.
Reviews of the field treat dead cells of lactic acid bacteria, including heat-killed Enterococcus faecalis, as a research subject of their own, separate from live probiotics (Taverniti and Guglielmetti, 2011; Adams, 2010). These are class-level reviews, not studies of this strain or of a finished product.
Start at the minimum order. Free sample. Specification, SDS and lot CoA on request.
Request a sample or quotationHeat-Killed Enterococcus faecalis Powder is a concentrated lactic acid bacteria ingredient for brands that declare a cell count rather than a live culture. Every gram carries at least 2.8 trillion heat-killed cells and nothing else: there is no dextrin or other carrier in the powder, so the formulator decides how the cells are diluted. As a calculation, 10 mg of powder carries at least 28 billion cells; the producer sets no recommended daily intake, and the dose of a finished product is set by the brand for its market.
The producer lists tablets, granules and soft capsules for supplements, and cosmetics, where the INCI name is Enterococcus Faecalis. The powder is stored at room temperature, so it needs no cold chain. The cells do not dissolve: in liquids they settle, and beauty drinks already on the market with this ingredient carry a "shake before use" instruction on the label.
Finished products can be filled and packed at our own Tomioka Plant in Gunma. Another fermentation-based ingredient in the range is Lactic Acid Fermented Barley Extract Powder, a heat-sterilized barley extract fermented with lactic acid bacteria.
Tell us the format you plan and we will say honestly what to expect. Request the specification, a quote or a sample.
This section explains the species, the terms used for inactivated bacteria and how the cells are counted. It is a class reference: it describes the material, not the results of studies on this strain, and we make no health claims from it.
Enterococcus faecalis is a lactic acid bacterium, a round (coccus-shaped) cell that is part of the normal gut flora. Enterococci occur in high numbers in some fermented cheeses and sausages, although they are not added deliberately as starter cultures, and some Enterococcus faecalis and Enterococcus faecium strains are used as probiotics, mostly in pharmaceutical preparations (Franz et al., 2011). The ingredient on this page is a single human-origin strain of the species, supplied as heat-killed cells.
Food microbiologists assess enterococci strain by strain: some strains, mostly hospital-adapted lineages, carry antibiotic-resistance or virulence traits, and the review literature concludes that the safety of each strain used in foods has to be assured individually (Franz et al., 2011). For your dossier, ask us early for the documents you need; the specification above lists what the producer's documents contain and what they do not.
Probiotics are by definition live microorganisms. For inactivated cells two terms are in use. In 2011 Taverniti and Guglielmetti proposed "paraprobiotic" for inactivated microbial cells or cell fractions, noting that non-viable cells could remove the shelf-life problems of live cultures (Taverniti and Guglielmetti, 2011). In 2021 an expert panel of the International Scientific Association for Probiotics and Prebiotics (ISAPP) defined a postbiotic as a "preparation of inanimate microorganisms and/or their components that confers a health benefit on the host" (Salminen et al., 2021). Under that definition the word applies to a product only when a health benefit has been shown for it, so on this page we describe the ingredient simply as heat-killed lactic acid bacteria.
Live probiotics are counted in colony-forming units (CFU), which only living cells produce. Heat-killed cells cannot form colonies, so this ingredient is specified by total cell count with DAPI, a fluorescent stain that binds DNA and makes every cell visible under the microscope, intact and inactivated alike. The figure of at least 2.8 trillion cells per gram is a DAPI count. The total plate count in the specification (max. 1,000 /g) is a separate hygiene parameter for viable microorganisms.
Reviews of the field treat dead cells of lactic acid bacteria, including heat-killed Enterococcus faecalis, as a research subject of their own, separate from live probiotics (Taverniti and Guglielmetti, 2011; Adams, 2010). These are class-level reviews, not studies of this strain or of a finished product.
No. It is heat-killed (inactivated) Enterococcus faecalis: the cells are heat-treated at 100 °C for 70 minutes before drying, so they are not live. The specification counts them as total cells by DAPI staining, at least 2.8 trillion per gram, and the powder is stored at room temperature.
100% heat-killed Enterococcus faecalis cells from a human-origin strain, with no carrier. The producer lists dipotassium hydrogen phosphate (culture medium, carry-over), sodium hydroxide (pH adjustment) and a silicone antifoam as processing aids. No allergenic substances are used. Corn is declared as non-segregated, so the ingredient is not offered as non-GMO.
No. Heat-killed bacterial cells do not dissolve, and in liquids they settle even at a low dose. The ingredient is used in finished beauty drinks, where the brand handles this with a "shake before use" instruction on the label. Tablets, granules and soft capsules are the supplement formats the producer lists.
The producer sets no recommended daily intake; the dose of a finished product is set by the brand for its market. As a calculation, 10 mg of powder carries at least 28 billion cells, because the specification guarantees a minimum of 2.8 trillion cells per gram.
